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This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.25-0.50 ug/ml overnight at 4C. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network which is funded by the National Cancer Institute.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.25-0.50 ug/ml overnight at 4C. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network which is funded by the National Cancer Institute.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.25-0.50 ug/ml overnight at 4C. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network which is funded by the National Cancer Institute.

FGF-basic Antibody [20B242.2]

CATALOG NUMBER: XP-5718-M

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant hFGF-basic as the immunizing antigen.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant human FGF-basic when used with our biotinylated antigen affinity purified anti-human FGF-basic (XP-5137Bt) as the detection antibody at a concentration of approximately 0.125-0.25 μg/mL.
Western blot:
To detect hFGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-basic is 1.0-2.0 ng/lane, under non-reducing conditions.
Purification:
This IgG1/K antibody was purified from a Protein A chromatography column.
Clonality:
Monoclonal
Physical State:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
FGF-basic lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Ncbi Official Symbol:
FGF2
Additional Names:
Fibroblast growth factor, FGFB, Fibroblast growth factor 2, Basic fibroblast growth factor, FGF-2, BFGF, HBGF-2
Protein Accession Number:
P09038
Protein Gi Number:
261260095
Ncbi Gene Id Number:
2247
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5718-M

List Size:
0.5 mg

List Price:

$355.00

Out of stock

Datasheet
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