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Western blot of whole cell T47D lysate prepared from cells that had been incubated in the presence of synthetic progestin agonist R5020(500nm) showing phosphospecific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser190.
Western blot of whole cell T47D lysate prepared from cells that had been incubated in the presence of synthetic progestin agonist R5020(500nm) showing phosphospecific immunolabeling of the ~90k PR-A isoform and the ~120 PR-B isoform of the progesterone receptor phosphorylated at Ser190.

Progesterone Receptor (phospho Ser190) Antibody [1154]

CATALOG NUMBER: XPS-1023

Clonality:
Monoclonal
Tested Applications:
IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Progesterone receptor (Ser190) monoclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphoSer190 of human progesterone receptor.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
As shown in Clemm, et al. (2000), cited above, the antibody specifically reacts with the progesterone receptor phosphorylated at Ser190 in T47D cells. Applications include Immunoblotting (IB), Immunohistochemistry (IHC) and Western Blots (WB). Initial recommended range of dilutions: 1:1000 to 1:2000. When internally tested under ideal conditions the working dilutions were 1:1000 for IB, IHC and WB.
Specificity:
Progesterone Receptor antibody is specific for the human progesterone receptor phosphorylated at Ser190.
Predicted Molecular Weight:
90/120
Purification:
Protein G purified
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Ncbi Official Symbol:
PGR
Protein Accession Number:
P06401
Protein Gi Number:
90110048
Ncbi Gene Id Number:
5241
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (A and B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones. Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor. Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.
Background Reference 1:
Clemm, D.L., Sherman, L., Boonyaratanakornkit, V., Schrader, W.T., Weigel, N.L., and Edwards, D.P., "Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody," Mol. Endocrinol., 14 (2000) 52 - 65.
Background Reference 2:
Lin, V. C. L., Woon, C. T., Aw, S. E., Guo, C., "Distinct molecular pathways mediate progesterone-induced growth inhibition and focal adhesion," Endocrinology 144 (2003) 5650 -5657.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1023

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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