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Tyrosine Hydroxylase, Sheep Antibody
Tyrosine Hydroxylase, Sheep Antibody

Tyrosine Hydroxylase Antibody

CATALOG NUMBER: XPS-2033

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Sheep
Species Reactivity:
Bovine, Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Sheep
Species Reactivity:
Bovine, Dog, Human, Mouse, Rat
Immunogen:
TH polyclonal antibody was raised against native rat tyrosine hydroxylase, purified from pheochromocytoma.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Tyrosine Hydroxylase antibody is specific for the 60k rat TH in lysates of PC-12 Cells. Applications include Immunofluorescence (IF), Immunohistochemistry (IHC) and Western Blot (WB). The antibody has been characterized in blot immunolabeling and immunocytochemical studies and cross-reacts with many different mammalian and some non-mammalian forms of TH. When internally tested under ideal conditions the working dilutions were 1:1000 for IHC, IF and WB.
Predicted Molecular Weight:
60
Purification:
Affinity Purified
Clonality:
Polyclonal
physical-state:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Ncbi Official Symbol:
Th
Protein Accession Number:
P04177
Protein Gi Number:
136577
Ncbi Gene Id Number:
25085
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons.
Background Reference 1:
Xu, Z.-Q., Lew, J. Y., Harada, K., Aman, K., Goldstein, M., Deutch, A., Haycock, J. W., and Hokfelt, T., "Immunohistochemical studies on phosphorylation of tyrosine hydroxylase in central catecholamine neurons using site- and phosphorylation state-specific antibodies," Neuroscience, 82 (1998) 727-738.
Background Reference 2:
Haycock, J.W. "Multiple forms of tyrosine hydroxylase in human neuroblastoma cells: Quantitation with isoform-specific antibodies," J. Neurochem. 60 (1993) 493-502.
Background Reference 3:
Haycock, J.W., "Four forms of tyrosine hydroxylase are present in human adrenal medulla," J. Neurochem. 56 (1991) 2139 - 2142.
Background Reference 4:
Haycock, J.W., "Quantitation of tyrosine hydroxylase protein levels: Spot immunolabeling with an affinity-purified antibody," Anal. Biochem. 181 (1989) 259 - 266.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-2033

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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