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Western blot analysis using AMACR monoclonal antibody against truncated AMACR - His recombinant protein and Jurkat cell lysate.
Immunohistochemical analysis of paraffin - embedded human prostate adenocarcinoma tissue showing cytoplasma location using AMACR antibody with DAB staining.
Western blot analysis using AMACR monoclonal antibody against truncated AMACR - His recombinant protein and Jurkat cell lysate.

AMACR Antibody [2A10F3]

CATALOG NUMBER: 32-107

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Purified truncated recombinant AMACR-His fusion protein expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG2b
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
AMACR monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AMACR
Additional Names:
2-methylacyl-CoA racemaseRM, RACE, CBAS4, AMACRD
Protein Accession Number:
Q9UHK6
Protein Gi Number:
313104070
Ncbi Gene Id Number:
23600
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.
Background Reference 1:
Chen Q. Watson JT. Marengo SR. et al. Cancer Lett. 2006, Dec 8, 244 (2):274-88.Epub 2006 Feb 23.
Background Reference 2:
Epstein JI. Herawi M. J Urol. 2006, Mar, 175 (3 Pt 1):820-34. Review.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-107

List Size:
0.1 mL

List Price:

$495.00

Datasheet
Shipping Info

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