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Immunohistochemical analysis of paraffin - embedded human normal lymphnode showing membrane localization using CD14 antibody with DAB staining
Flow cytometic analysis of Human PBMC using monoclonal antibody to human CD14.
Immunohistochemical analysis of paraffin - embedded human normal lymphnode showing membrane localization using CD14 antibody with DAB staining

CD14 Antibody [5A3H5D5(FC) / 5A3B11B5]

CATALOG NUMBER: 32-128

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified recombinant human CD14 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
IHC:Dilution 1:200 - 1:400
ELISA:Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein G affinity chromatography and resuspended in PBS with 0.2%BSA,
0.09% sodium azide. No reconstitution is necessar Concentration is 0.4mg/ml.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
CD14 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD14
Additional Names:
Myeloid cell-specific leucine-rich glycoprotein
Protein Accession Number:
P08571
Protein Gi Number:
20141203
Ncbi Gene Id Number:
929
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD14 antigen is a GPI-linked glycoprotein with a molecular weight of 55kD. The CD14 antigen is expressed on cells of the myelomonocytic lineage including monocytes, macrophages and Langerhans cells. Low expression is observed on neutrophils and on human B cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding protein (LBP). LBP and CD14 antigen serves two physiological roles. These proteins act as opsonin and opsonic receptor, respectively, to promote the phagocytic uptake of bacteria or LPScoated particles by macrophages.
Background Reference 1:
J Mammary Gland Biol Neoplasia 2000, 5 : 227-241.
Background Reference 2:
J Mammary Gland Biol Neoplasia 2000, 5 : 165-185.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-128

List Size:
0.1 mL

List Price:

$408.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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