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Western blot analysis using CD31 monoclonal antibody against truncated CD31 recombinant protein.
Immunohistochemical analysis of paraffin - embedded human lymphonodus tissue showing cytoplasmic location of vascular endothelial cells using CD31 antibody with DAB staining.
Western blot analysis using CD31 monoclonal antibody against truncated CD31 recombinant protein.

CD31 Antibody [2F7B2]

CATALOG NUMBER: 32-131

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
CD31 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PECAM1
Additional Names:
EndoCAM, PECAM-1, CD31, PECA1, GPIIA', endoCAM, CD31/EndoCAM
Protein Accession Number:
P16284
Protein Gi Number:
129747
Ncbi Gene Id Number:
5175
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors.It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells.The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1.It is also weakly expressed on many peripheral lymphoid cells and platelets.CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
Background Reference 1:
Mayr U et al. Circ Res 98:412-20 (2006).
Background Reference 2:
Bingle L et al. Br J Cancer 94:101-7 (2006).
Background Reference 3:
Wynne F et al. Reproduction 131:721-32 (2006).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-131

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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