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Western blot analysis using RSK1 monoclonal antibody against truncated RSK1 - His recombinant protein.
Immunohistochemical analysis of paraffin - embedded human colon adenocarcinoma (top) and human liver carcinoma (bottom) showing nuclear location using RSK1 antibody with DAB staining.
Western blot analysis using RSK1 monoclonal antibody against truncated RSK1 - His recombinant protein.

RSK1 Antibody [10B1D7A9 / 10B1D7E12]

CATALOG NUMBER: 32-212

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant RSK1 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG2a
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
RSK1 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RPS6KA1
Additional Names:
MAPKAPK1A, RSK1, 90 kDa ribosomal protein S6 kinase 1, S6K-alpha-1, RSK, HU-1, MAPKAPK1A
Protein Accession Number:
Q15418
Protein Gi Number:
20178306
Ncbi Gene Id Number:
6195
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Rsk1 is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine / threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB. RSK proteins possess two separate kinase domains, the C terminal kinase domain and the N terminal kinase domain, separated by a linker region containing a hydrophobic motif. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock, and irradiation.
Background Reference 1:
Alcorta, D.A., et al. 1989. Mol. Cell. Biol. 9:3850-3859.
Background Reference 2:
Sweet, L.J., et al. 1990. Mol. Cell. Biol. 10: 2413-2417.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-212

List Size:
0.1 mg

List Price:

$432.00

Datasheet
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