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Western blot analysis using SNCG monoclonal antibody against truncated SNCG recombinant protein.
Immunohistochemical analysis of paraffin - embedded human ovary carcinoma (top) and breast carcinoma (bottom) showing cytoplasmic (ovary carcinoma) and cytoplasmic / nuclear (breast carcinoma) location using SNCG antibody with DAB staining.
Western blot analysis using SNCG monoclonal antibody against truncated SNCG recombinant protein.

SNCG Antibody [1H10D2]

CATALOG NUMBER: 32-216

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant SNCG expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
SNCG (breast cancer-specific protein 1) monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SNCG
Additional Names:
BCSG1, PERSYN, PRSN, Breast cancer-specific gene 1 protein, SR, BCSG1
Protein Accession Number:
O76070
Protein Gi Number:
90110074
Ncbi Gene Id Number:
6623
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SNCG(also designated gamma-synuclein or breast cancer-specific protein 1),with 127-amino acid protein(about 14 kDa), belongs to the synuclein family, which also includes alpha- and beta- synuclein.Three synucleins are located in the neuronal cytosol and enriched in presynaptic terminals,while SNCG is also expressed in many other non-neuronal tissues. SNCG is abnormally expressed in a high percentage of tumor tissues of diversified cancer types, including liver, esophagus, colon, gastric, lung, prostate, cervical, and breast cancer, but rarely expressed in tumor-matched nonneoplastic adjacent tissues. High levels of SNCG have been identified in advanced breast carcinomas suggesting a correlation between overexpression of SNCG and breast tumor development.
Background Reference 1:
Haiyan Liu, Wei Liu, Yinwei Wu. Cancer Res. 2005 Sep 1;65(17):7635-43.
Background Reference 2:
rina Surgucheva , Belinda McMahon , Andrei Surguchov. Cell Motil Cytoskeleton. 2006 May 26.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-216

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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