Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis using Trim5a monoclonal antibody against truncated Trim5αrecombinant protein.
Western blot analysis using Trim5a monoclonal antibody against breast tissue lysate with Pierce SuperSignal Febto substrate (top). Immunohistochemical analysis of paraffin - embedded human metastatic adenocarcinoma (middle) and stomach adenocarcinoma (bottom) showing cytoplasmic localization using Trim5a antibody with DAB staining.
Western blot analysis using Trim5a monoclonal antibody against truncated Trim5αrecombinant protein.

Trim5a Antibody [3B11H2]

CATALOG NUMBER: 32-231

Clonality:
Monoclonal
Tested Applications:
IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant human trim5 alpha expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot:Dilution 1:1,000 - 1:5,000
IHC(P):Dilution 1:100 - 1:400
IHC(F):Dilution 1:200 - 1:400
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
Trim5α monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZCCHC3
Additional Names:
C20orf99C20orf99
Protein Accession Number:
Q9NUD5
Protein Gi Number:
23396534
Ncbi Gene Id Number:
85364
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRIM5-alpha is a protein that is found in the cells of many mammals and fends of various retrovirus infections. It protects monkeys from infection with HIV-1, and humans from infection with some other viruses. If a retrovirus has entered a cell, it needs to shed its capsid in order to reversely transcribe its genes, so that they can be expressed by the host cell. It is believed that TRIM5α, which is present in the cytoplasm, somehow recognizes the capsid and blocks its shedding, thereby stopping the virus in its tracks. It thus represents an intracellular defense completely separate from the rest of the body's immune system.
Background Reference 1:
Stremlau, M. Nature 2004.427:848-53.
Background Reference 2:
Song, B. J Virol. 2005.79(7):3930-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-231

List Size:
0.1 mL

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369