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Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antigen (5 ug/mL) and serial dilutions of antibody. <br><br>E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antigen (5 ug/mL) and serial dilutions of antibody.

E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.

NDUFA10 Antibody

CATALOG NUMBER: XW-7881

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Chicken
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human
Immunogen:
21-140
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
NADH-ubiquinone oxidoreductase 42 kDa subunit, mitochondrial antibody can be used for the detection of NADH-ubiquinone oxidoreductase 42 kDa subunit, mitochondrial by ELISA and Western Blot, may also work for ICC and IHC.
Predicted Molecular Weight:
40.8 kDa (calculated)
Purification:
Immunoaffinity Purified
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Phosphate-Buffered Saline. No preservatives added.
Concentration:
1 mg/mL
Storage Conditions:
NDUFA10 antibody can be stored at 4°C for short term (weeks). Long term storage should be at -20°C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
NDUFA10
Additional Names:
Complex I-42kD, CI-42kD, NADHubiquinone oxidoreductase 42 kDa subunit, mitochondrial, Complex I-42KD, CI-42KD
Protein Accession Number:
NP_004535.1
Protein Gi Number:
4758768
Ncbi Gene Id Number:
4705
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FUNCTION: Transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

CATALYTIC ACTIVITY: NADH + acceptor = NAD+ + reduced acceptor.

SUBUNIT: Mammalian complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction.

COFACTOR: Binds 1 FAD per subunit.

SUBCELLULAR LOCATION: Mitochondrion; mitochondrial matrix.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

XW-7881

List Size:
0.05 mg

List Price:

$638.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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