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Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF using IκB-α (Phospho-Ser32/Ser36).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-α (Phospho-Ser32/Ser36).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using IkappaB-α (Phospho-Ser32/Ser36).
Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF using IκB-α (Phospho-Ser32/Ser36).

IkB a (phospho Ser32 / Ser36) Antibody

CATALOG NUMBER: 79-175

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IkB-a (Phospho-Ser32/Ser36) antibody was raised against a peptide sequence around phosphorylation site of serine 32/36 (H-D-S (p) -G-L-D-S (p) -M-K) derived from Human IkappaB-α.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of IkappaB-α only when phosphorylated at serine 32/36.
Predicted Molecular Weight:
39 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
NFKBIA
Additional Names:
IKBA, MAD-3, NFKBI, ADDA, I-kappa-B-alpha, IKBA, NF-kappaB inhibitor alpha, NFKBIA
Protein Accession Number:
NP_065390.1
Protein Gi Number:
10092619
Ncbi Gene Id Number:
4792
Background:
Inhibits the activity of dimeric NF-κ-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.
Background Reference 1:
Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823. Honda, R. et al. (1997) FEBS Lett. 420, 25-27. Hirao, A. et al. (2000) Science 287, 1824-1827. Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157. Chehab, N. H. et al. (1999) Proc . Natl . Acad. Sci. USA 96, 13777-13782.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-175

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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