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Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using Src (Phospho-Tyr529).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src (Phospho-Tyr529).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using Src (Phospho-Tyr529).
Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using Src (Phospho-Tyr529).

Src (phospho Tyr529) Antibody

CATALOG NUMBER: 79-176

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Src (Phospho-Tyr529) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y (p) -Q-P) derived from Human Src.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of Src only when phosphorylated at tyrosine 529.
Predicted Molecular Weight:
60 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
SRC
Additional Names:
ASV, SRC1, c-SRC, p60-Src, PKB, RAC, C-SRC
Protein Accession Number:
NP_005408.1
Protein Gi Number:
4885609
Ncbi Gene Id Number:
6714
Background:
proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.
Background Reference 1:
Pyper J. M., (1985) Mol. Cell. Biol. 5:831-838Pyper J. M. (1990) Mol. Cell. Biol. 10:2035-2040Xu W., (1997) . Nature 385:595-602Benes C. H., (2005) Cell 121:271-280

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-176

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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