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Western blot analysis of lysed extracts from various cells using ASK1 (Phospho-Ser966).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1 (Phospho-Ser966).
Western blot analysis of lysed extracts from various cells using ASK1 (Phospho-Ser966).

ASK1 (phospho Ser966) Antibody

CATALOG NUMBER: 79-187

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
ASK1 (Phospho-Ser966) antibody was raised against a peptide sequence around phosphorylation site of serine 966 (S-I-S (p) -L-P) derived from Human ASK1.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of ASK1 only when phosphorylated at serine 966.
Predicted Molecular Weight:
155 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
MAP3K5
Additional Names:
ASK1, MEKK5, MAPKKK5, SRK, ASK-1, M3K5, MAP3K5, MAPK/ERK kinase kinase 5
Protein Accession Number:
NP_005914.1
Protein Gi Number:
5174547
Ncbi Gene Id Number:
4217
Background:
Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
Background Reference 1:
Zhang W, et al. (2005) J Biol Chem. 280 (19): 19036-19044. Fujii K, et al. (2004) Oncogene. 23 (29): 5099-5104. Goldman EH, et al. (2004) J Biol Chem 2004 Mar 12; 279 (11): 10442-10449. Zhang L, et al. (1999) Proc Natl Acad Sci U S A. 96 (15): 8511-8515.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-187

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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