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Western blot analysis of lysed extracts from HL60 cells using Zap-70 (Ab-319).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Zap-70 (Ab-319).
Western blot analysis of lysed extracts from HL60 cells using Zap-70 (Ab-319).

Zap 70 (Ab 319) Antibody

CATALOG NUMBER: 79-347

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Zap-70 (Ab-319) antibody was raised against a peptide sequence around aa.317~321 (S-P-Y-S-D) derived from Human ZAP70.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of total Zap-70 protein.
Predicted Molecular Weight:
70 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
ZAP70
Additional Names:
SRK, STD, TZK, STCD, ZAP-70, NFKB3, 70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70
Protein Accession Number:
NP_001070.2
Protein Gi Number:
31455611
Ncbi Gene Id Number:
7535
Background:
ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the ζ chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-ζ chain, which can mediate the transduction of extracellular stimuli into cellular effector functions
Background Reference 1:
Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100 (2): 443-448Ku GM, et al. (2001) EMBO J; 20 (3): 457-465Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96 (17): 9775-9780Zhao Q, et al. (1996) Mol Cell Biol; 16 (12): 6765-6774Williams BL, et al. (1999) EMBO J; 18 (7): 1832-1844Adjali O, et al. (2005) J Clin Invest; 115 (8): 2287-2295.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-347

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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