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Western blot analysis of lysed extracts from 293 cells untreated or treated with hydroxyurea using Cyclin E1 (phospho-Thr395).
Western blot analysis of lysed extracts from 293 cells untreated or treated with hydroxyurea using Cyclin E1 (phospho-Thr395).

Cyclin E1 (phospho Thr395) Antibody

CATALOG NUMBER: 79-510

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Cyclin E1 (phospho-Thr395) antibody was raised against a peptide sequence around phosphorylation site of threonine 395 (L-L-T (p) -P-P) derived from Human Cyclin E1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:1000
Specificity:
This antibody detects endogenous level of Cyclin E1 only when phosphorylated at threonine 395.
Predicted Molecular Weight:
48 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CCNE1
Additional Names:
CCNE, SIGLEC2, CCNE, CCNE1
Protein Accession Number:
NP_001229.1
Protein Gi Number:
17318559
Ncbi Gene Id Number:
898
Background:
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition.
Background Reference 1:
Won K. A., Reed S. I. EMBO J. 15:4182-4193 (1996) Welcker M., Singer J., Loeb K. R., Grim J., Bloecher A., Mol. Cell 12:381-392 (2003)

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-510

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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