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Western blot analysis of lysed extracts from HeLa and 293 cells using H2B Antibody.
Western blot analysis of lysed extracts from HeLa and 293 cells using H2B Antibody.

H2B Antibody

CATALOG NUMBER: 79-638

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
H2B antibody was raised against a peptide sequence around aa.41~45 (Y-V-Y-K-V) derived from Human H2B.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous level of total H2B protein.
Predicted Molecular Weight:
14 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
HIST2H2BE
Additional Names:
H2B, H2BQ, GL105, H2B.1, H2BFQ, H2BGL105
Protein Accession Number:
NP_003519.1
Protein Gi Number:
4504277
Ncbi Gene Id Number:
8349
Background:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Background Reference 1:
Borchert GM, et al. (2010) PLoS One. 5 (7): e11641. Pennini ME, et al. (2010) PLoS Pathog. 6 (7): e1000995. Lu C, et al. (2010) Sci China Life Sci. 53 (6): 663-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-638

List Size:
0.1 mg

List Price:

$370.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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