Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of extracts from Hela cells treated with serum using BTK (phospho-Tyr223) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from Hela cells treated with serum using BTK (phospho-Tyr223) Antibody. The lane on the right is treated with the antigen-specific peptide.

BTK (phospho Tyr223) Antibody

CATALOG NUMBER: 79-779

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
BTK (phospho Tyr223) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 223 (A-L-Y (p)-D-Y) derived from Human BTK.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous levels of BTK only when phosphorylated at tyrosine 223.
Predicted Molecular Weight:
80 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
BTK
Additional Names:
AGMX1, ATK, kinase Btk, BPK
Protein Accession Number:
NP_000052.1
Protein Gi Number:
547759
Ncbi Gene Id Number:
695
Background:
Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK.
Background Reference 1:
Sulekha Verma, J. Biol. Chem., Feb 2001; 276: 4671 - 4676.
Background Reference 2:
Yan M. Li, Cancer Res., Nov 2003; 63: 7630 - 7633.
Background Reference 3:
Malti Nikrad, Mol. Cancer Ther., Mar 2005; 4: 443 - 449.
Background Reference 4:
Feng Dong, Infect. Immun., Mar 2005; 73: 1861 - 1864.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-779

List Size:
0.1 mg

List Price:

$376.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369