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Western blot analysis of extracts from HepG2 cells treated with PMA using WWOX (Phospho-Tyr33) Antibody. The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using WWOX (Phospho-Tyr33) antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from HepG2 cells treated with PMA using WWOX (Phospho-Tyr33) Antibody. The lane on the right is treated with the antigen-specific peptide.

WWOX (phospho Tyr33) Antibody

CATALOG NUMBER: 79-889

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
WWOX (phospho Tyr33) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 33 (W-V-Y (p)-Y-A) derived from Human WWOX.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
The antibody detects endogenous levels of WWOX only when phosphorylated at tyrosine 33.
Predicted Molecular Weight:
55 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
WWOX
Additional Names:
FOR, WOX1, Aberrant WW domain-containing oxidoreductase
Protein Accession Number:
NP_057457.1
Protein Gi Number:
7706523
Ncbi Gene Id Number:
51741
Background:
WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). The highest normal expression of this gene is detected in hormonally regulated tissues such as testis, ovary, and prostate. This expression pattern and the presence of an SRD domain suggest a role for this gene in steroid metabolism.
Background Reference 1:
Bednarek A.K., Cancer Res. 60:2140-2145(2000).
Background Reference 2:
Ried K., Hum. Mol. Genet. 9:1651-1663(2000).
Background Reference 3:
Bednarek A.K., Cancer Res. 61:8068-8073(2001).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-889

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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