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Western blot analysis of extracts from K562 cells using Smad2 (Phospho-Ser465) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from K562 cells using Smad2 (Phospho-Ser465) Antibody. The lane on the right is treated with the antigen-specific peptide.

Smad2 (phospho Ser465) Antibody

CATALOG NUMBER: 79-947

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Smad2 (phospho Ser465) antibody was raised against a peptide sequence around phosphorylation site of Serine 465 (C-S-S (p)-M-S) derived from Human Smad2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous levels of Smad2 only when phosphorylated at serine 465.
Predicted Molecular Weight:
52 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
SMAD2
Additional Names:
hMAD-2, JV18, JV18-1, MAD, hSMAD2
Protein Accession Number:
NP_001003652.1
Protein Gi Number:
51173730
Ncbi Gene Id Number:
4087
Background:
Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
Background Reference 1:
Riggins G.J., Nat. Genet. 13:347-349(1996).
Background Reference 2:
Zhang Y., Nature 383:168-172(1996).
Background Reference 3:
Eppert K., Cell 86:543-552(1996).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-947

List Size:
0.1 mg

List Price:

$376.00

Datasheet
Shipping Info

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