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Western blot analysis of extracts from HepG2 cells treated with Forskolin using Phospho-CDK1/CDC2 (Thr14) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from HepG2 cells treated with Forskolin using Phospho-CDK1/CDC2 (Thr14) Antibody. The lane on the right is treated with the antigen-specific peptide.

CDK1/CDC2 (phospho Thr14) Antibody

CATALOG NUMBER: 80-045

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CDK1/CDC2 (phospho Thr14) antibody was raised against a peptide sequence around phospho rylation site of threonine14 (E-G-T (p)-Y-G) derived from Human CDK1/CDC2 .
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of CDK1/CDC2 only when phosphorylated at threonine 14.
Predicted Molecular Weight:
34 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CDK2
Additional Names:
CDC28, CDC2A, CDK1, MPF, kinase Cdc2
Protein Accession Number:
NP_001277159.1
Protein Gi Number:
16936528
Ncbi Gene Id Number:
1017
Background:
Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus.
Background Reference 1:
Sarkar S, Dey BK, Dutta A (2010) Mol Biol Cell 21, 2138-49
Background Reference 2:
Shreeram S, Hee WK, Bulavin DV (2008) Mol Cell Biol 28, 7442-50
Background Reference 3:
Mayya V, et al. (2006) Mol Cell Proteomics 5, 1146-57

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

80-045

List Size:
0.1 mg

List Price:

$355.00

Out of stock

Datasheet
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