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Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Erk1/2-BIOT (Cat. No. 12080-08). Proteins were visualized using Mouse Anti-BIOT-HRP (Cat. No. 99-182) secondary antibody and chemiluminescent detection.
Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Erk1/2-BIOT (Cat. No. 12080-08). Proteins were visualized using Mouse Anti-BIOT-HRP (Cat. No. 99-182) secondary antibody and chemiluminescent detection.

Biotin Antibody [SB58c] (HRP)

CATALOG NUMBER: 99-182

Clonality:
Monoclonal
Tested Applications:
Flow, ICC, IHC-Fr, IHC-P, IHC-WM, WB
Host Species:
Mouse
Conjugate:
HRP
Specifications
Host Species:
Mouse
Immunogen:
Biotin-KLH
Conjugate:
HRP
Tested Applications:
Flow, ICC, IHC-Fr, IHC-P, IHC-WM, WB
Application Note:
Biotin Antibody [SB58c] for use in ELISA and western blotting assays.
Specificity:
Biotin
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
Supplied in a stock solution of 50% glycerol/50% PBS, pH 7.4; No preservative added
Concentration:
batch dependent
Storage Conditions:
Store vial at 2-8°C
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Anti-Biotin may be used for indirect immunostaining when working with a biotinylated primary antibody. As an effective alternative to avidin, Anti-Biotin amplifies signal and reduces background that can be caused by avidin and its derivatives.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

99-182

List Size:
1 mL

List Price:

$408.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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