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Total cell lysates from NIH/3T3 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bax-UNLB (Cat. No. 99-623). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP secondary antibody and chemiluminescent detection.
Total cell lysates from NIH/3T3 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Bax-UNLB (Cat. No. 99-623). Proteins were visualized using Goat Anti-Mouse IgG, Human ads-HRP secondary antibody and chemiluminescent detection.

Bax Antibody [6A7]

CATALOG NUMBER: 99-623

Clonality:
Monoclonal
Tested Applications:
Flow
Host Species:
Mouse
Species Reactivity:
Bovine, Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Bovine, Human, Monkey, Mouse, Rat
Immunogen:
Bax peptide (a.a. 12-24) common to mouse, rat, and human
Conjugate:
Unconjugated
Tested Applications:
Flow
Application Note:
Bax Antibody [6A7] for use in flow cytometry, immunohistochemistry / immunocytochemistry, electron microscopy, western blotting, immunoprecipitation, and neutralization assays.
Specificity:
Bax
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration:
0.1 mg/mL
Storage Conditions:
Store vial at 2-8°C
Ncbi Official Symbol:
BAX
Additional Names:
Apoptosis regulator BAX, BCL2 associated X protein
Protein Accession Number:
Q07812
Ncbi Gene Id Number:
581;12028;24887
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bax is a 20-22 kDa member of the Bcl-2 family of proteins involved in regulation of programmed cell death, or apoptosis. In murine thymocytes, it is expressed primarily as a cytoplasmic protein. Over-expression of Bax promotes apoptosis by formation of homodimers and through heterodimerization with Bcl-2, an inhibitor of apoptosis. The monoclonal antibody 6A7 reacts with human, mouse and rat Bax but does not bind the soluble cytosolic form of Bax; however, treatment of cells with non-ionic detergents exposes the epitope and allows binding of 6A7 to monomeric forms of Bax but not Bax complexed with either Bcl-2 or Bcl-xL.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

99-623

List Size:
0.1 mg

List Price:

$391.00

Datasheet
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