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<strong>Figure 1 ELISA Validation with Spike Trimer Proteins of SARS-CoV-2 Variants and SARS-CoV-1 </strong><br>Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, SD9789. A direct ELISA was performed using Spike trimer protein of SARS-CoV-2 Variants (Delta and Omicron) and SARS-CoV-1 as coating antigens at 1 μg/mL and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (SD9789) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 16 ng/mL to 2000 ng/mL.
<strong>Figure 2 Epitope Mapping of four Spike S2 Antibodies by Direct Competitive ELISA </strong><br>A direct competitive ELISA was developed for the epitope mapping using Spike trimer proteins of SARS-CoV-2 Delta variant at 1 μg/ml as the coating antigen and biotinylated Spike S2 single domain antibodies (sdAbs) as the competitive antibodies. The sdAb mixtures (biotinylated and unconjugated sdAbs) were added to ELISA plate and streptavidin-HRP was used to detect the captured biotinylated sdAbs. The assay suggests the antibodies bind to different epitopes of the Spike trimer.
Figure 1 ELISA Validation with Spike Trimer Proteins of SARS-CoV-2 Variants and SARS-CoV-1
Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, SD9789. A direct ELISA was performed using Spike trimer protein of SARS-CoV-2 Variants (Delta and Omicron) and SARS-CoV-1 as coating antigens at 1 μg/mL and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (SD9789) as the capture antibody, following by anti-cMyc-tag antibody (PM-7669) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 16 ng/mL to 2000 ng/mL.
Recombinant
Trial Size

SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1G5]

CATALOG NUMBER: SD9789

Clonality:
Recombinant Monoclonal
Tested Applications:
ELISA
Host Species:
Llama
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Llama
Species Reactivity:
Virus
Immunogen:
SARS-CoV-2 Spike S2 protein containing C-terminal His Tag. The protein was expressed in human 293 cells (HEK293). It contains amino acids Ser 686 - Pro 1213.
Conjugate:
Unconjugated
Tested Applications:
ELISA
Purification:
SARS-CoV-2 (COVID-19) Spike S2 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.  It also contains a myc-tag for detection.
Clonality:
Recombinant Monoclonal
Isotype:
sdAb
physical-state:
Liquid
Buffer:
SARS-CoV-2 (COVID-19) Spike S2 Antibody is supplied in PBS.
Concentration:
batch dependent
Storage Conditions:
SARS-CoV-2 (COVID-19) Spike S2 Antibody should be stored in working aliquots at -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
S
Additional Names:
SARS-CoV-2 (COVID-19) Spike S2 Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
Protein Accession Number:
QHD43416
Protein Gi Number:
1791269090
Ncbi Gene Id Number:
43740568
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
As SARS-CoV-2 Spike S2 protein is more conserved among coronaviruses than Spike S1 protein, antibodies against spike S2 protein may react with more Coronaviruses, serving as a pan-coronvirus antibody. The functional characteristics analysis revealed that P1G5 has excellent binding activity to spike trimer of SARS-CoV-1, and SARS-CoV-2 variants (delta and omicron). Biotinylated P1G5 can still bind spike trimer of SARS-CoV-2. In addition, P1G5 and another three spike S2 sdAbs (P1A6, P1A9, and P1B8) belong to different families based on their sequence alignment, and epitope mapping experiment indicated that they may bind to different epitopes with different affinity to the different strains in the binding ELISA. Our data showed that these spike S2 sdAbs bind spike trimer of both SARS-CoV-2 and SARS-CoV-1, suggesting that these spike S2 sdAbs have potentials as a novel and efficient tool suitable for pan-coronavirus studies as well as for identification of various coronaviruses and their variants from clinical samples.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

SD9789

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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