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<strong>Figure 1 Western Blot Validation in Mouse and Rat Cell Lines </strong><br>Loading: 15 μg of lysates per lane. Antibodies: PGAM5, 9517 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines </strong><br>Loading: 15 μg of lysates per lane. Antibodies: PGAM5, 9517 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse Tissues </strong><br>Loading: 15 μg of lysates per lane. Antibodies: PGAM5, 9517 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4 Western Blot Validation in Rat Tissues </strong><br>Loading: 15 μg of lysates per lane. Antibodies: PGAM5, 9517 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 5 Immunofluorescence Validation of PGAM5 in Human Liver</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver labeling PGAM5 with 9517 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 6 Immunofluorescence Validation of PGAM5 in Mouse Testis</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling PGAM5 with 9517 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 7 Immunofluorescence Validation of PGAM5 in Rat Testis</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PGAM5 with 9517 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 1 Western Blot Validation in Mouse and Rat Cell Lines
Loading: 15 μg of lysates per lane. Antibodies: PGAM5, 9517 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
New
Trial Size

PGAM5 (CT) Antibody

CATALOG NUMBER: 9517

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: gorilla (100%); Chimpanzee (100%); monkey (100%).
Immunogen:
Anti-PGAM5 antibody (9517) was raised against a peptide corresponding to 14 amino acids near the carboxyl terminus of human PGAM5.

The immunogen is located within the last 50 amino acids of PGAM5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
WB: 0.5 μg/mL; IF: 10-20 μg/mL.

Antibody validated: Western Blot in human, mouse, and rat samples; Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1203 – A549 Cell Lysate
Positive Control 2:
Cat. No. 1282 – 3T3/NIH Cell Lysate
Positive Control 3:
Cat. No. 1403 – Mouse Brain Lysate
Predicted Molecular Weight:
Predicted: 32kD

Observed: 32 kD
Isoforms:
Human PGAM5 has 2 isoforms, including isoform 1 (289aa, 32kD), and isoform 2 (255aa, 28kD). Mouse PGAM5 has 2 isoforms, including isoform 1 (288aa, 32kD) and isoform 2 (287aa, 31.9kD). Rat PGAM5 has one isoform (288aa, 32kD). 9517 can detect PGAM5 in human, mouse and rat
Purification:
PGAM5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PGAM5 Antibody is supplied in PBS containing 0.02% sodium azide
Concentration:
1 mg/mL
Storage Conditions:
PGAM5 antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PGAM5
Additional Names:
Serine/threonine-protein phosphatase PGAM5 mitochondrial, Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5
Protein Accession Number:
Q96HS1
Protein Gi Number:
150417955
Ncbi Gene Id Number:
192111
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PGAM5 Antibody: PGAM5 is a central mediator for programmed necrosis induced by TNF and may be a regulator of mitochondrial dynamics. It displays phosphatase activity for serine/threonine residues, dephosphorylates and activates MAP3K5 kinase. It has apparently no phosphoglycerate mutase activity and is a substrate for a KEAP1-dependent ubiquitin ligase complex. It is also contributes to the repression of NFE2L2-dependent gene expression
Background Reference 1:
Lo S. et al. Exp. Cell Res. 2018; 314(8): 1789-1803
Background Reference 2:
Takeda et al. PNAS 2009; 106(30): 12301-12305
Background Reference 3:
Want et al. Cell 2012; 148(1-2); 228-243

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9517

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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