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<strong>Figure 1 Western Blot Validation In Mouse Tissues </strong><br>Loading: 15 &#956g of lysates per lane. Antibodies: IRGB10 9515, 2 &#956g/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Immunofluorescence Validation of IRGB10 in Mouse Spleen </strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen labeling IRGB10 with 9515 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 1 Western Blot Validation In Mouse Tissues
Loading: 15 μg of lysates per lane. Antibodies: IRGB10 9515, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
New
Trial Size

IRGB10 (CT) Antibody

CATALOG NUMBER: 9515

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Monkey (100%), Gorilla (100%)
Immunogen:
Anti-IRGB10 antibody (9515) was raised against a peptide corresponding to 13 amino acids near the C-terminus of mouse IRGB10.

The immunogen is located within the last 50 amino acids of IRGB10.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
WB: 2 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1409 – Mouse Thymus Lysate
Positive Control 2:
Cat. No. 1411 – Mouse Colon Lysate
Predicted Molecular Weight:
Predicted: 47kD

Observed: 47 kD
Isoforms:
Mouse IRGB10 has 1 isoform (417aa, 47.2kD).
Purification:
IRGB10 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IRGB10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IRGB10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IRGB10
Additional Names:
IRGB10 Antibody: Immunity-related GTPase family member b10, Interferon-gamma-inducible p47 GTPase, Gm12250, OTTMUSG00000005723
Protein Accession Number:
AGY29631
Protein Gi Number:
551032829
Ncbi Gene Id Number:
631323
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IRGB10 Antibody: IRGB10 is an inferno-inducible protein that is essential for the activation of DNA-sensing AIM2 inflammasome, and contributes to the activation of LPS-sensing caspase-11 and NLRP3 inflammasome. IRGB10 is part of a conserved signaling hub at the interface between cell-autonomous immunity and innate immune sensing pathways. IRGB10 targets cytoplasmic bacteria and compromises bacterial structural integrity. IRGB10 and LIGP2 act together to confer differential susceptibility against Chlamydia infection in mice.
Background Reference 1:
Man et al. Cell 2016; 167(2):382-396.e17
Background Reference 2:
Bernstein-Hanley I et al. Proc Natl Acad Sci USA. 2006;103(38):14092-14097
Background Reference 3:
Miyairi I et al. J Immunol 2007; 179(3):1814-1824
Background Reference 4:
Coers J et al. J Immunol 2008; 180(9):6237-6245

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9515

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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