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<strong>Figure 1 WB Validation in Human, Mouse and Rat Cell Lines</strong><br>Loading: 15 μg of lysate. Antibodies: FZD6 9551, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Immunofluorescence Validation of FZD6 in Daudi Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling FZD6 with 9551 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 3 Immunofluorescence Validation of FZD6 in Mouse Lung</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung labeling FZD6 with 9551 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 4 Immunofluorescence Validation of FZD6 in Rat Lung</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling FZD6 with 9551 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 1 WB Validation in Human, Mouse and Rat Cell Lines
Loading: 15 μg of lysate. Antibodies: FZD6 9551, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
New
Trial Size

FZD6 (IN) Antibody

CATALOG NUMBER: 9551

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chimpanzee(100%); Monkey (100%); Dog (100%)
Immunogen:
Anti-FZD6 antibody (9551) was raised against a peptide corresponding to 14 amino acids in the central domain of human FZD6.
The immunogen is located within 50-550 amino acids of FZD6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
WB: 2 μg/mL; IF: 10 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1219 - MCF-7 Cell Lysate
Positive Control 2:
Cat. No. 1282 - 3T3/NIH Cell Lysate
Predicted Molecular Weight:
Predicted: 79kD
Observed: 95 kD (Post-translational modification: 2 N-linked glycosylations)
Isoforms:
Human FZD6 has 2 isoforms, including isoform 1 (706aa, 79.3kD) and isoform 2 (674aa, 75.6kD). Mouse FZD6 has 1 isoform (709aa, 79kD). Rat FZD6 has one isoform (710aa, 79.1kD). 9551 can detect FZD6 in human, mouse and rat.
Purification:
FZD6 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FZD6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FZD6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FZD6
Additional Names:
FZD6 Antibody: Frizzled-6, Fz-6, hFz6
Protein Accession Number:
O60353
Protein Gi Number:
215273986
Ncbi Gene Id Number:
8323
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FZD6 Antibody: FZD6 acts as a receptor for Wnt proteins. Frizzled receptors are coupled to the beta-catenin canonical signaling pathway. Together with FZD3, FZD6 is involved with neural tube closure and plays a role in the regulation of planar cell polarity, particularly in the orientation of asymmetric bundles of stereocilia on apical faces of a subset of sensory cells located in the inner ear.
Background Reference 1:
Wang et al. J. Neurosci. 206;26(8):2147-2156

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9551

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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