Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

<b>Blocking of Fc-receptors by anti-CD16/32 antibody.</b> <i>Ex vivo</i> murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate- left and middle panel) as well as bone marrow-derived macrophages (BMDMs- right panel) were stained with a murine anti-human antibody (fluorescently lAF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (24-122- clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently lntibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.
<b> Anti-CD16/32 staining of murine macrophages</b> Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD16/32 antibody 24-122 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor® 647(AF647) commercially avail a competitor. Compared to the isotype control and secondary-only control- a CD16/32-positive population is apparent.
Blocking of Fc-receptors by anti-CD16/32 antibody. Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate- left and middle panel) as well as bone marrow-derived macrophages (BMDMs- right panel) were stained with a murine anti-human antibody (fluorescently lAF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (24-122- clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently lntibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.

CD16/CD32 Antibody [2.4G2], Rat IgG2b

CATALOG NUMBER: 24-122

Clonality:
Recombinant Monoclonal
Tested Applications:
Blocking, Flow, IF, IHC, IP
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Mouse
Species:
Rat
Original Species Name:
Rat
Immunogen:
Mouse BALB/c Macrophage J774 Cell Line.
Conjugate:
Unconjugated
Tested Applications:
Blocking, Flow, IF, IHC, IP
Clonality:
Recombinant Monoclonal
Isotype:
IgG2b kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
Fcgr2b
Additional Names:
Fcγ III/II Receptor, Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, CD16, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB, FcRII IgG Fc rec
Ncbi Gene Id Number:
14130
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-122

List Size:
0.2 mg

List Price:

$598.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369