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<b> Flow cytometry using the Anti-CLU antibody SAIC-43B-8 ( </b> Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of SAIC-43B-8 (24-340- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Western Blot using anti-CLU antibody SAIC-43B-8 ( </b> HeLa(A) (0.03ug/ml)- MCF7(B) (0.01ug/ml) cell lysate and human brain cerebellum(C) (0.03ug/ml)- human plasma (D) (0.03ug/ml) and human testes(E) (0.03ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-43B-8 (24-340) before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
<b> Immunofluorescence staining of HeLa cells with anti-CLU (SAIC-43B-8 </b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-43B-8 (24-340) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-340- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-CLU antibody SAIC-43B-8 ( Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of SAIC-43B-8 (24-340- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

CLU Antibody [SAIC-43B-8], Rabbit IgG

CATALOG NUMBER: 24-340

Clonality:
Recombinant Monoclonal
Tested Applications:
ELISA, immunoMRM, WB
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human
Species:
Rabbit
Original Species Name:
Rabbit
Immunogen:
Peptide "ASSIIDELFQDR" derived from Clusterin conjugated to KLH. Clusterin isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins
Conjugate:
Unconjugated
Tested Applications:
ELISA, immunoMRM, WB
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
CLU
Additional Names:
Clusterin, APOJ, CLI, KUB1, AAG4
Ncbi Gene Id Number:
1191
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-340

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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