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<b> Flow-cytometry using the anti-MHC II antibody P7/7 ( </b> Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r1 version of P7/7 (24-540- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> Immunofluorescence staining of mouse splenocytes using anti-MHC II antibody (P7/7. </b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of P7/7 (24-540) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-540- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.
Flow-cytometry using the anti-MHC II antibody P7/7 ( Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control- black line) or the r1 version of P7/7 (24-540- blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

MHC II Antibody [P7/7], Rabbit IgG

CATALOG NUMBER: 24-540

Clonality:
Recombinant Monoclonal
Tested Applications:
Flow, IHC, IP
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Mouse, Rat
Species:
Rabbit
Original Species Name:
Rat
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
This antibody was raised by immunising DA rats with PVG.R8 rat spleen cells. Splenocytes from immunised rats were then isolated, and fused to the myeloma line NSO/u to generate stable hybridomas.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC, IP
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Additional Names:
P7.7, I-A, Ia, I-E, MHC-II, MHC Class II
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-540

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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