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<b> Immunofluorescence staining of fixed RAW264.7 cells with anti-4-1BBL antibody AT113-2 (</b> Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of AT113-2 (24-611) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-611- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Flow cytometry using the anti- 4-1BBL antibody AT113-2 ( </b> RAW264.7 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r1 version of AT113-2 (24-611- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of fixed RAW264.7 cells with anti-4-1BBL antibody AT113-2 ( Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of AT113-2 (24-611) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-611- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

4-1BBL Antibody [AT113-2], Rabbit IgG

CATALOG NUMBER: 24-611

Clonality:
Recombinant Monoclonal
Tested Applications:
Blocking, Flow, WB
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Mouse
Species:
Rabbit
Original Species Name:
Rat
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
This antibody was raised by immunising LOU rats with soluble recombinant 4-1BBL:Fc fusion protein.
Conjugate:
Unconjugated
Tested Applications:
Blocking, Flow, WB
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
Tnfsf9
Additional Names:
CD137L, TNFSF9, 4-1BB ligand, 41BBL, CDw137L, homolog of mouse 4-1BB-L, receptor 4-1BB ligand, tumor necrosis factor (ligand) superfamily member 9, tumor necrosis factor (ligand) superfamily, member 9, tumor necrosis factor ligand 5A, Tumor necrosis factor ligand superfamily member 9.
Ncbi Gene Id Number:
21950
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-611

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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