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<b> Immunofluorescence staining of fixed mouse splenocytes with anti-GITR antibody DTA-1 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of DTA-1 (24-621) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-621- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Flow cytometry using the Anti-GITR antibody DTA-1 ( </b> Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of DTA-1 (24-621- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of fixed mouse splenocytes with anti-GITR antibody DTA-1 ( Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of DTA-1 (24-621) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-621- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

GITR Antibody [DTA-1], Rabbit IgG

CATALOG NUMBER: 24-621

Clonality:
Recombinant Monoclonal
Tested Applications:
Agonism, Flow, IP, WB
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Mouse
Species:
Rabbit
Original Species Name:
Rat
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
This antibody was raised by immunising Wistar rats with the CD25+CD4+ T cell line. Hybridoma cells were generated by fusing spleen cells from the immunized rat with P3X63Ag8.653 myeloma cells, assessed for secreting antibodies capable of neutralizing in vitro CD25+CD4+ T cell-mediated suppression of anti-CD3-stimulated T cell proliferation9, and then cloned. This DTA-1 clone secreted a monoclonal rat immunoglobulin G2a (IgG2a) that showed high neutralizing activity.
Conjugate:
Unconjugated
Tested Applications:
Agonism, Flow, IP, WB
Clonality:
Recombinant Monoclonal
Isotype:
IgG lambda
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
Tnfrsf18
Additional Names:
CD357, AITR, GITR-D, Glucocorticoid-induced TNFR-related protein, TNFRSF18, Tumor necrosis factor receptor superfamily member 18
Ncbi Gene Id Number:
21936
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-621

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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