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<b> Immunofluorescence staining of fixed mouse splenocytes with anti-CD25 antibody PC-61.5.3 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of PC-61.5.3 (24-631) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-631- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
Immunofluorescence staining of fixed mouse splenocytes with anti-CD25 antibody PC-61.5.3 ( Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version of PC-61.5.3 (24-631) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-631- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

CD25 Antibody [PC-61.5.3], Rabbit IgG

CATALOG NUMBER: 24-631

Clonality:
Recombinant Monoclonal
Tested Applications:
Depletion, ELISA, Flow, Functional Assays, IF, WB
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Mouse
Species:
Rabbit
Original Species Name:
Rat
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
This antibody was raised by immunising rats with the B6.1 mouse cytotoxic T cell line, followed by fusing spleen cells with P3X63Ag8.653 myeloma cells.
Conjugate:
Unconjugated
Tested Applications:
Depletion, ELISA, Flow, Functional Assays, IF, WB
Clonality:
Recombinant Monoclonal
Isotype:
IgG lambda
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
Il2ra
Additional Names:
IL2-RA, IL-2 receptor subunit alpha,L-2R alpha chain, IL-2R subunit alpha, Interleukin-2 receptor subunit alpha, p55, p55 chain, PC61, PC6153
Ncbi Gene Id Number:
16184
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-631

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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