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<b> Immunofluorescence staining of fixed K562 cells with anti-Rhesus D antigen antibody D7C2 (</b> Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric r version of D7C2 (24-654) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-654- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Flow cytometry using the Anti-Rhesus D antigen antibody D7C2 ( </b> Paraformaldehyde fixed K562 cells were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of D7C2 (24-654- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of fixed K562 cells with anti-Rhesus D antigen antibody D7C2 ( Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric r version of D7C2 (24-654) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-654- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Rhesus D antigen Antibody [D7C2], Rabbit IgG

CATALOG NUMBER: 24-654

Clonality:
Recombinant Monoclonal
Tested Applications:
ADCC, Flow
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human
Species:
Rabbit
Original Species Name:
Human
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
A Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
Conjugate:
Unconjugated
Tested Applications:
ADCC, Flow
Clonality:
Recombinant Monoclonal
Isotype:
IgG lambda
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
RHD
Additional Names:
CD240D, RHXIII, Rh polypeptide 2, RhPII
Ncbi Gene Id Number:
6007
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-654

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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