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<b> Immunofluorescence staining of fixed A431 cells with anti-CD63 antibody NK-1-C3 (</b> Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric r version of NK-1-C3 (24-762) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-762- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Western Blot using anti-CD63 antibody NK-1-C3 (</b> K562 cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of NK-1-C3 (24-762) at 0.1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Immunofluorescence staining of fixed A431 cells with anti-CD63 antibody NK-1-C3 ( Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric r version of NK-1-C3 (24-762) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-762- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

CD63 Antibody [NK-1-C3], Rabbit IgG

CATALOG NUMBER: 24-762

Clonality:
Recombinant Monoclonal
Tested Applications:
Flow, IF, IHC, IP, WB
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Human, Mouse
Species:
Rabbit
Original Species Name:
Mouse
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
This antibody was raised by immunizing BALB/c mice with a plasma membrane preparation of the human melanoma MeWo cells.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, IP, WB
Clonality:
Recombinant Monoclonal
Isotype:
IgG kappa
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
CD63
Additional Names:
Granulophysin, LAMP-3, Tetraspanin-30, Tspan-30, Lysosomal-associated membrane protein 3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, CD63 antigen, NK1/C3, NKI/C3, NK-1.C3, NK1C3
Ncbi Gene Id Number:
967
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-762

List Size:
0.2 mg

List Price:

$598.00

Datasheet
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