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<b> Western Blot using anti-CD79b antibody HM79-16 (</b> Mouse spleen tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of HM79-16 (24-774) at 0.1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
<b> Immunofluorescence staining of fixed mouse splenocytes with anti-CD79b antibody HM79-16 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ coverslips stained with the chimeric r version HM79-16 (24-774) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-774- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.
<b> Flow cytometry using the Anti-CD79b antibody HM79-16 ( </b> Paraformaldehyde fixed mouse splenocytes were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of HM79-16 (24-774- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Western Blot using anti-CD79b antibody HM79-16 ( Mouse spleen tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of HM79-16 (24-774) at 0.1 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

CD79b Antibody [HM79-16], Rabbit IgG

CATALOG NUMBER: 24-774

Clonality:
Recombinant Monoclonal
Tested Applications:
Flow, IF, IHC, IP
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Species Reactivity:
Mouse
Species:
Rabbit
Original Species Name:
Hamster, Armenian
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Armenian Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
Immunogen:
HM79-16 was initially generated by purifying CD79b from WEHI231 B cells and used for immunisation of footpads of an Armenian hamster. Hybridoma supernatants were screened for positive reactivity in surface immunofluorescence of WEHI231 and then the specificity to CD79b was tested by immuno-precipitationRecombinant anti-CD79 antibodies were engineered by cloning and sequencing the full-length IG heavy and light chains of an Armenian hamster (Cricetulus migratorius).
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC, IP
Clonality:
Recombinant Monoclonal
Isotype:
IgG lambda
Buffer:
PBS with 0.02% Proclin 300.
Concentration:
batch dependent
Storage Conditions:
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Ncbi Official Symbol:
Cd79b
Additional Names:
Ig-beta, AGM6, B29, IGB, CD79b molecule,
Ncbi Gene Id Number:
15985
User Note:
For research use only.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-774

List Size:
0.2 mg

List Price:

$663.00

Datasheet
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