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NT-3 Antibody

CATALOG NUMBER: 38-305

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNT-3. Anti-Human NT-3 specific antibody was purified by affinity chromatography employing immobilized hNT-3 matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Sandwich ELISA:To detect hNT-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human NT-3 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNT-3.
Western Blot
To detect hNT-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
NT-3 specific antibody was purified by affinity chromatography employing immobilized hNT-3 matrix
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
NTF3
Additional Names:
Neurotrophin-3, Neurotrophic factor, Nerve Growth Factor-2 (NGF-2), HGNF
Protein Accession Number:
P20783
Protein Gi Number:
P20783.1
Ncbi Gene Id Number:
4908
User Note:
Centrifuge vial prior to opening.
Background:
NT-3 is a neurotrophic factor structurally related tobeta-NGF, BDNF, and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-3 is expressed by neurons of the central nervous system, and can signal through the trk receptors. NT-3 promotes the growth and survival of nerve and glial cells. The amino acid sequences of human, murine and rat NT-3 are identical. Recombinant Human NT-3 is a noncovalently linked homodimer of two 13.6 kDa polypeptide monomers (240 total amino acid residues).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

38-305

List Size:
0.05 mg

List Price:

$391.00

Datasheet
Shipping Info

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