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To detect Human sFas Receptor by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Human sFas Receptor as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human sFas Receptor.
To detect Human sFas Receptor by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Human sFas Receptor as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human sFas Receptor.

sFas Receptor Antibody

CATALOG NUMBER: 38-346

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Human sFas Receptor. Anti-Human sFas Receptor specific antibody was purified by affinity chromatography employing an immobilized Human sFas Receptor matrix.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Sandwich ELISA: To detect Human sFas Receptor by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human sFas Receptor as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human sFas Receptor.
Western Blot
To detect Human sFas Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human sFas Receptor is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
sFas Receptor specific antibody was purified by affinity chromatography employing an immobilized Human sFas Receptor matrix
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
FAS
Additional Names:
soluble Fas receptor (sFasR), TNFRSF6, CD95, Apo I, Fas Antigen
Protein Accession Number:
P25445
Protein Gi Number:
P25445.1
Ncbi Gene Id Number:
355
User Note:
Centrifuge vial prior to opening.
Background:
Fas and Fas Ligand (FasL) belong to the TNF superfamily, and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD, followed by processing of the pro-enzyme into active forms. These active caspases then cleave various cellular substrates, leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and a 145 amino acid cytoplasmic domain. Recombinant Human soluble Fas (sFas Receptor) is a 157 amino acid polypeptide (17.6 kDa) corresponding to the TNFR-homologous cysteine-rich extracellular Fas domain.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

38-346

List Size:
0.05 mg

List Price:

$391.00

Datasheet
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