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<strong>Figure 1 WB Validation in Human Cell Lines </strong><br>Loading: 15 μg of human cell lysate Antibodies: PPARGC1A 7793, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>WB Validation in Human Lung </strong><br>Loading: 15 μg of human lung lysate Antibodies: PPARGC1A 7793, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 WB Validation in Mouse Skin </strong><br>Loading: 15 μg of mouse skin lysate Antibodies: PPARGC1A 7793, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 WB Validation in Rat Kidney </strong><br>Loading: 15 μg of rat kidney lysate Antibodies: PPARGC1A 7793, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunofluorescence Validation of PPARGC1A in MCF7 Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling PPARGC1A with 7793 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 6 Immunofluorescence Validation of PPARGC1A in HELA Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PPARGC1A with 7793 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 7 Immunohistochemistry Validation of PPARGC1A in Human Colon Carcinoma Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PPARGC1A antibody (7793) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 8 Immunohistochemistry Validation of PPARGC1A in Human Pancreas Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PPARGC1A antibody (7793) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 9 Immunohistochemistry Validation of PPARGC1A in Mouse Stomach Tissue </strong><br>Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PPARGC1A antibody (7793) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Flow Cytometry Validation of PPARGC1A in HeLa Cells 1X10^6/mL HeLa cells were intracellularly stained with 2 μg/mL of PPARGC1A antibody (red) or Rabbit IgG isotype control (black) at RT for 30 min, followed by PSFlo 488 conjugated goat anti-rabbit IgG secondary antibody staining (1:1000) at RT for 30 min. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100 in PBS.
Figure 1 WB Validation in Human Cell Lines
Loading: 15 μg of human cell lysate Antibodies: PPARGC1A 7793, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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PPARGC1A Antibody

CATALOG NUMBER: 7793

Clonality:
Polyclonal
Tested Applications:
ELISA, FC, IHC-P, WB, ICC/IF
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (94%)
Immunogen:
PPARGC1A antibody was raised against a 17 amino acid peptide near the amino terminus of human PPARGC1A.
The immunogen is located within amino acids 200 - 250 of PPARGC1A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IHC-P, WB, ICC/IF
Application Note:
WB: 2 μg/mL; IHC-P: 1-2 μg/mL; ICC/IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse and rat samples; Immunofluorescence and Flow Cytometry in human samples. All other applications and species not yet tested.
Specificity:
PPARGC1A antibody is human, mouse and rat reactive. At least three isoforms of PPARGC1A are known to exist; this antibody will detect all three isoforms.
Positive Control 1:
Cat. No. 1419 - Mouse Skin Tissue Lysate
Positive Control 2:
Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 3:
Cat. No. 1224 - Daudi Cell Lysate
Positive Control 4:
Cat. No. 1204 - K562 Cell Lysate
Predicted Molecular Weight:
Predicted: 91kD
Observed: 100 kD
Purification:
PPARGC1A antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PPARGC1A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PPARGC1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
PPARGC1A
Additional Names:
PPARGC1A Antibody: LEM6, PGC1, PGC1A, PGC-1v, PPARGC1, PGC-1(alpha), LEM6, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, Ligand effect modulator 6, PGC-1-alpha
Protein Accession Number:
NP_037393
Protein Gi Number:
7019499
Ncbi Gene Id Number:
10891
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A), also known as LEM-6, is a transcriptional coactivator that regulates the genes involved in energy metabolism (1). PPARGC1A interacts with PPARgamma, which permits the interaction of PPARGC1A with multiple transcription factors. PPARGC1A can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination (2). PPARGC1A may be also involved in the development of obesity (3).
Background Reference 1:
Tsuemi T and La Spada AR. PGC-1a at the intersection of bioenergetics regulation and neuron function: from Huntington's disease to Parkinson's disease and beyond. Prog. Neurobiol. 2012; 97:142-51.
Background Reference 2:
Kang C and Li Ji L. Role of PGC-1a signaling in skeletal muscle health and disease. Ann. NY Acad. Sci. 2012; 1271:110-7.
Background Reference 3:
Liu C and Lin JD. PGC-1 coactivators in the control of energy metabolism. Acta Biochim. Biophys. Sin. 2011; 43:248-57.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7793

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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