Western blot analysis of lysates from wild type(WT) and CDX2 knockdown(KD) 293T cells, using [KD Validated] CDX2 Rabbit mAb at1:500 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

Western blot analysis of lysates from Mouse colon, using [KD Validated] CDX2 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.

Western blot analysis of lysates from SW620 cells, using [KD Validated] CDX2 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.

Western blot analysis of lysates from wild type(WT) and CDX2 knockdown(KD) 293T cells, using [KD Validated] CDX2 Rabbit mAb (A20222) at1:500 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10s.

Western blot analysis of lysates from Mouse colon, using [KD Validated] CDX2 Rabbit mAb (A20222) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 30s.

Western blot analysis of lysates from SW620 cells, using [KD Validated] CDX2 Rabbit mAb (A20222) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10s.

Western blot analysis of lysates from wild type(WT) and CDX2 knockdown(KD) 293T cells, using [KD Validated] CDX2 Rabbit mAb (A20222) at1:500 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 10s.

[KD Validated] CDX2 Rabbit Recombinant Antibody (81-682)

CATALOG NUMBER: 81-682

Clonality:

Monoclonal

Tested Applications:

ELISA, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Immunogen:

Synthetic peptide. This information is considered to be commercially sensitive.

Conjugate:

Unconjugated

Tested Applications:

ELISA, WB

Application Note:

WB,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Predicted Molecular Weight:

34kDa

Advanced Validation

Properties

purification:

Affinity purification

Clonality:

Monoclonal

Isotype:

IgG

physical-state:

Liquid

Buffer:

Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Concentration:

Batch dependent

Storage Conditions:

Store at -20℃. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

CDX2

Additional Names:

CDX3; CDX-3; CDX2/AS; X2

Protein Accession Number:

Q99626

Ncbi Gene Id Number:

1045

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

This product is for research use only.

View product citations for catalog number 81-682 on CiteAb

CATALOG NUMBER:

81-682

List Size:

100 uL

List Price:

$540.00

Datasheet

Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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[KD Validated] CDX2 Rabbit Recombinant Antibody (81-682)

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

purification:

Clonality:

Isotype:

physical-state:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

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