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Western blot analysis of lysates from HeLa cells, using HDAC2 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using HDAC2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using HDAC2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using HDAC2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using HDAC2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using HDAC2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using HDAC2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using HDAC2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using HDAC2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using HDAC2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of C6 cells using HDAC2 Rabbit mAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using HDAC2 Rabbit mAb at dilution of 1:100 (40xlens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300μg extracts from HeLa cells using 3μg HDAC2 Rabbit mAb. Western blot was performed from the immunoprecipitate using HDAC2 Rabbit mAb at a dilution of 1:500.
Western blot analysis of lysates from HeLa cells, using HDAC2 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.

HDAC2 Rabbit Recombinant Antibody (82-253)

CATALOG NUMBER: 82-253

Clonality:
Monoclonal
Tested Applications:
ChIP, ELISA, IF/ICC, IHC-P, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ChIP, ELISA, IF/ICC, IHC-P, IP, WB
Application Note:
WB,1:500 - 1:2000, IHC-P,1:200 - 1:800, IF/ICC,1:50 - 1:200, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements., ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements., ChIP,5μg antibody for 10μg-15μg of Chromatin.
Positive Control 1:
HeLa
Predicted Molecular Weight:
55kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HDAC2
Additional Names:
HD2; RPD3; YAF1; KDAC2; HDAC2
Protein Accession Number:
Q92769
Ncbi Gene Id Number:
3066
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-253

List Size:
100 uL

List Price:

$540.00

Datasheet
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