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Confocal imaging of paraffin-embedded Human malignant melanoma tissue using Tyrosinase Rabbit mAb (A21267, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Confocal imaging of paraffin-embedded Human malignant melanoma tissue using Tyrosinase Rabbit mAb (A21267, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Tyrosinase Rabbit Recombinant Antibody

CATALOG NUMBER: 81-982

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-P
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-P
Application Note:
IF-P,1:50 - 1:200, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
60kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TYR
Additional Names:
ATN; CMM8; OCA1; OCA1A; OCAIA; SHEP3; Tyrosinase
Protein Accession Number:
P14679
Ncbi Gene Id Number:
7299
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-982

List Size:
100 uL

List Price:

$477.00

Datasheet
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