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Western blot analysis of lysates from Neuro-2a cells, using TET3 Rabbit mAb (A21930) at 1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with TET3 using TET3 Rabbit mAb (A21930) at 1:20000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020) | Exposure time: 30 s.
Western blot analysis of lysates from Neuro-2a cells, using TET3 Rabbit mAb (A21930) at 1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.

TET3 Rabbit Recombinant Antibody

CATALOG NUMBER: 82-014

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:2000 - 1:20000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Neuro-2a,293T transfected with TET3
Predicted Molecular Weight:
195kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Tet3
Additional Names:
B430006D22Rik; D230004J03Rik; TET3
Protein Accession Number:
Q8BG87
Ncbi Gene Id Number:
194388
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables methylcytosine dioxygenase activity. Involved in DNA demethylation of male pronucleus. Acts upstream of or within DNA demethylation. Located in cytoplasm; female pronucleus; and male pronucleus. Is expressed in several structures, including central nervous system; early conceptus; future brain; and genitourinary system. Orthologous to human TET3 (tet methylcytosine dioxygenase 3).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-014

List Size:
100 uL

List Price:

$477.00

Datasheet
Shipping Info

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