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Western blot analysis of various lysates using TWF2 Rabbit mAb (A22727) at 1:20000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 180 s.
Western blot analysis of lysates from Jurkat cells using TWF2 Rabbit mAb (A22727) at 1:8000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 20 s.
Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg TWF2 Rabbit mAb(A22727). Western blot was performed from the immunoprecipitate using TWF2 Rabbit mAb(A22727) at a dilution of 1:10000.
Western blot analysis of various lysates using TWF2 Rabbit mAb (A22727) at 1:20000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 180 s.

TWF2 Rabbit Recombinant Antibody

CATALOG NUMBER: 82-364

Clonality:
Monoclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
WB,1:2000 - 1:20000, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
40kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TWF2
Additional Names:
A6r; A6RP; PTK9L; MSTP011; TWF2
Protein Accession Number:
Q6IBS0
Ncbi Gene Id Number:
11344
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-364

List Size:
100 uL

List Price:

$477.00

Datasheet
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