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Western blot analysis of lysates from 293F cells, using DMT1/SLC11A2 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.
Immunofluorescence analysis of 293T cells using DMT1/SLC11A2 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of 293F cells using 3 μg DMT1/SLC11A2 Rabbit mAb. Western blot was performed from the immunoprecipitate using DMT1/SLC11A2 Rabbit mAb antibody at a dilition of 1:1000.
Western blot analysis of lysates from 293F cells, using DMT1/SLC11A2 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.

DMT1/SLC11A2 Rabbit Recombinant Antibody (82-577)

CATALOG NUMBER: 82-577

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, IP, WB
Application Note:
WB,1:500 - 1:1000, IF/ICC,1:50 - 1:200, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
61KDa/62KDa/64KDa/65KDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SLC11A2
Additional Names:
DCT1; DMT1; AHMIO1; NRAMP2; DMT1/SLC11A2
Protein Accession Number:
P49281
Ncbi Gene Id Number:
4891
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the solute carrier family 11 protein family. The product of this gene transports divalent metals and is involved in iron absorption. Mutations in this gene are associated with hypochromic microcytic anemia with iron overload. A related solute carrier family 11 protein gene is located on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-577

List Size:
100 uL

List Price:

$540.00

Datasheet
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