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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Orexin B using Orexin B Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: () at 1:10000 dilution. | Lysates/proteins: 20 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit | .Exposure time: 90s.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Orexin B using Orexin B Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: () at 1:10000 dilution. | Lysates/proteins: 20 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit | .Exposure time: 90s.

Orexin B Rabbit Recombinant Antibody (83-406)

CATALOG NUMBER: 83-406

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
293F
Predicted Molecular Weight:
13kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Orexin B/Hypocretin-2
Additional Names:
OX; PPOX; NRCLP1
Protein Accession Number:
O43612
Ncbi Gene Id Number:
3060
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-406

List Size:
100 uL

List Price:

$477.00

Datasheet
Shipping Info

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