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Western blot analysis of lysates from Oryza sativa, using Actin(Plant Specific) Rabbit mAb at 1:10000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.
Western blot analysis of Arabidopsis thaliana, using Actin(Plant Specific) Rabbit mAb at 1:10000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.
Western blot analysis of lysates from Oryza sativa, using Actin(Plant Specific) Rabbit mAb at 1:10000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.

Actin(Plant Specific) Rabbit Recombinant Antibody (82-810)

CATALOG NUMBER: 82-810

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Oryza sativa, Widely Range
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Arabidopsis thaliana, Oryza sativa, Widely Range
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:10000 - 1:60000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Oryza sativa,Arabidopsis thaliana
Predicted Molecular Weight:
45kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ACT12
Additional Names:
ACT12; Actin(Plant Specific)
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Actin is an ancient conserved protein that is encoded by multiple isovariants in multi-cellular organisms. There are eight functional actin genes in the Arabidopsis genome.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-810

List Size:
100 uL

List Price:

$204.00

Datasheet
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