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Western blot analysis of lysates from GSDMD knockout (KO) HeLa cells and GSDMD knockout (KO) HeLa cells transfected with LPS using Cleaved IL-18 (Tyr37) Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time:60s. | <b>WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS</b>
Western blot analysis of Recombinant Human IL-18 Protein using Cleaved IL-18 (Tyr37) Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 1 ng per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 60s.
Western blot analysis of lysates from GSDMD knockout (KO) HeLa cells and GSDMD knockout (KO) HeLa cells transfected with LPS using Cleaved IL-18 (Tyr37) Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time:60s. | WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Cleaved IL-18 (Tyr37) Rabbit Recombinant Antibody (82-854)

CATALOG NUMBER: 82-854

Clonality:
Monoclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB,1:1000 - 1:2000
Positive Control 1:
GSDMD knockout (KO) HeLa cells transfected with LPS,Recombinant Human IL-18 Protein
Predicted Molecular Weight:
22KDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IL-18
Additional Names:
mature IL-18; Cleaved IL-18 (Tyr37)
Protein Accession Number:
Q14116
Ncbi Gene Id Number:
3606
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a proinflammatory cytokine of the IL-1 family that is constitutively found as a precursor within the cytoplasm of a variety of cells including macrophages and keratinocytes. The inactive IL-18 precursor is processed to its active form by caspase-1, and is capable of stimulating interferon gamma production, and of regulating both T helper (Th) 1 and Th2 responses. This cytokine has been implicated in the injury of different organs, and in potentially fatal conditions characterized by a cytokine storm. In humans, IL-18 gene is located on chromosome 11. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-854

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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