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Western blot analysis of lysates from THP-1 cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (A24059) at 1:72000 dilution incubated overnight at 4℃. THP-1 cells were treated with PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 45s.
Western blot analysis of lysates from wild type (WT) and Cleaved Gasdermin D knockout (KO) THP-1 cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (A24059) at 1:72000 dilution incubated at room temperature for 1.5 hours. THP-1 cells and Gasdermin D knockout (KO) THP-1 cells were treated with PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 20s.
Confocal imaging of THP-1 cells (treated with PMA and LPS) and THP-1 cells (untreated) cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (A24059, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Western blot analysis of lysates from THP-1 cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (A24059) at 1:72000 dilution incubated overnight at 4℃. THP-1 cells were treated with PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 45s.

[KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit Recombinant Antibody

CATALOG NUMBER: 82-855

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, WB
Application Note:
WB,1:70000 - 1:280000, IF/ICC,1:100 - 1:400, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
THP-1 treated with LPS and PMA
Predicted Molecular Weight:
53kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GSDMD
Additional Names:
DF5L; DFNA5L; FKSG10; GSDMDC1
Protein Accession Number:
P57764
Ncbi Gene Id Number:
79792
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-855

List Size:
100 uL

List Price:

$540.00

Datasheet
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