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Flow cytometry: 1X10^6 T-47D cells (negative control,left) and RPMI 8226 cells (right) were surface-stained with CD177 Rabbit mAb or PSFlo 647 Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 RPMI 8226 cells were surface-stained with PSFlo 647 Rabbit IgG isotype control or CD177 Rabbit mAb(2 μg/mL,right).
Flow cytometry: 1X10^6 T-47D cells (negative control,left) and RPMI 8226 cells (right) were surface-stained with CD177 Rabbit mAb or PSFlo 647 Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

CD177 Rabbit Recombinant Antibody (83-060)

CATALOG NUMBER: 83-060

Clonality:
Monoclonal
Tested Applications:
ELISA, FC
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC
Application Note:
FC,1:100 - 1:500, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
15kDa/27kDa/46kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CD177
Additional Names:
CD177; HNA-2a; HNA2A; NB1; NB1 GP; PRV-1; PRV1; NB1GP; CD177 antigen
Protein Accession Number:
Q8N6Q3
Ncbi Gene Id Number:
57126
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-060

List Size:
100 uL

List Price:

$477.00

Datasheet
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