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Western blot analysis of various lysates, using TOMM40 Rabbit mAb (A24644) at 1:4800 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using TOMM40 Rabbit mAb (A24644) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver using TOMM40 Rabbit mAb (A24644) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon using TOMM40 Rabbit mAb (A24644) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using TOMM40 Rabbit mAb (A24644) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver using TOMM40 Rabbit mAb (A24644) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Confocal imaging of HeLa cells using TOMM40 Rabbit mAb (A24644,at dilution of 1:300) (red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Confocal imaging of NIH/3T3 cells using TOMM40 Rabbit mAb (A24644,at dilution of 1:300) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Immunoprecipitation of TOMM40 from 500 µg extracts of Hep G2 cells was performed using 2 µg of TOMM40 Rabbit mAb (A24644). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using TOMM40 Rabbit mAb (A24644) at a dilution of 1:5000.
Western blot analysis of various lysates, using TOMM40 Rabbit mAb (A24644) at 1:4800 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 5s.

TOMM40 Rabbit Recombinant Antibody

CATALOG NUMBER: 82-967

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, IHC-P, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, IHC-P, IP, WB
Application Note:
WB,1:2000 - 1:10000, IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells, IHC-P,1:100 - 1:500, IF/ICC,1:100 - 1:500, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
38kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TOMM40
Additional Names:
TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E; TOMM40
Protein Accession Number:
O96008
Ncbi Gene Id Number:
10452
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is localized in the outer membrane of the mitochondria. It is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for import of protein precursors into mitochondria. Alternatively spliced transcript variants have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-967

List Size:
100 uL

List Price:

$477.00

Datasheet
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